® BioFlo 110 Fermentor & Bioreactor You’ll love it for its mind. NEW BRUNSWICK SCIENTIFIC Where Quality and Innovation Have Become Tradition The most intelligent modular fermentor on earth. With the BioFlo 110, New Brunswick Scientific expands the fermentation universe. Every configuration of this autoclavable fermentation and cell culture system — from modest entry point to fully loaded — is built around a controller so intelligent that using it takes little more than common sense. Add exceptional power and cost-effectiveness, and the BioFlo1 10 is not just the smartest choice, but very probably the only choice. With the Gas Mix Controller, blend Easily change the setpoint or 4-gasses to gently regulate pH & graph range dO , or choose 100% of one gas 2 for probe calibration Intelligent design. We’ve done some ordinary things in extraordinary ways: • Self-illuminated display provides bright text and graphics • Removable plug at the end of the sparge ring facilitates cleaning • O-ring at the bottom — not the top — of each port prevents contamination from reaching the vessel • Heat blankets with two large cut-outs and a reflective white inner surface make it easy to view the culture in non-jacketed vessels • Water-jacketed vessels provide gentle, uniform temperature control in a space-saving design • Slip-on motor self-aligns with drive shaft. No pre-alignment needed • Complete vessel assemblies include correctly-sized rotameters • Dished-bottom vessels have no mixing dead zones, even at low agitation speeds • pH and DO probes can be adjusted to any immersion Out of the length box and ready to go. • Controller changes The BioFlo 110 is surprisingly easy to set up, and from “Fermentation” equally easy to expand as your needs grow. The to most basic kit includes a Primary Control Unit “Cell Culture” and Power Controller to manage temperature mode with a and agitation. Addi-tional control modules single touch are recognized automatically — no configu- • Original factory ration required. settings can be easily recalled Of course the BioFlo 110 controller also commu- nicates with computers. Use one of our ® BioCommand software packages to automate data collection and supervise your cultures. You won’t easily outgrow the BioFlo 110’scapabilities. NEW BRUNSWICK SCIENTIFIC Where Quality and Innovation Have Become Tradition Select “Fermentation” or “Cell Culture” mode, and one of four display languages Meet the mind behind the machine. The controller at the heart of the BioFlo 110 fermentor is a leap to a new level of user- friendliness. Its big, bright display uses plain English (or plain French, German or Spanish) to clearly show you what’s going on in the vessel. Calling up a graph or changing a set point is just a touch away. Perfectly natural and intuitive to use, the BioFlo 110 is designed to let you concentrate on your culture. In making it easy to use, we did not forget about capability. Growing mammalian cells? Bacteria or yeast? Alternating? No problem. One touch lets you instantly tailor control responses to suit the applica- tion. Want to add a second vessel? A third or fourth? The 32-loop BioFlo 110 Primary Control Unit supports up to four independent vessels and their loop con- trollers. Simultaneously. Advanced users can change P-I-D values and cascade options, choose dead-band or P-I-D control — always with the knowledge that you can return to factory settings quickly and easily. Advanced users may modify control response by altering “dead-band” and control gains Features. • Choose temperature control via water-jacketed vessels (shown above) or heat blanket • User can select P-I-D or adjustable dead-band control for pH • Adjustable P-I-D values for pH and Dissolved Oxygen • One Primary Control Unit controls up to four vessels • Built-in PCU floppy-drive makes future firmware upgrades “do-it-yourself” simple • Computer-ready with built-in BioCommand and Modbus serial protocols • Clean, reliable surface seals provided in direct drive agitation systems • Optional magnetic drives for critical cell culture applications Decisions, decisions, decisions… made easy. To make the BioFlo 110 as easy to buy as it is to use, we pre-configured several complete fermen- tation and cell culture kits. Very competitively- priced basic kits include automatic agitation and temperature controls, along with manual airflow. Advanced kits add pH/DO, 4-gas, foam controls and more. Individual components can also be added at any time to meet your changing needs. So, let us show off our brainchild. The moment you take the controls, you’ll see why the BioFlo 110 is the intelligent choice. BIOFLO 110 FERMENTOR/BIOREACTOR SPECIFICATIONS * Total Capacity 1.3 Liters 3 Liters 7.5 Liters 14 Liters Working Volume 0.4 - 1.0 Liters 0.8 - 2.2 Liters 2.0 - 5.6 Liters 4.0 - 10.5 Liters Water-jacketed and heat-blanketed vessels available VESSEL Design All are autoclavable, dished-bottom, borosilicate glass Heat-blanketed vessels include integral bench and autoclave stand Stand Water-jacketed vessels are self-standing Fermentation Direct drive, 50 - 1,200 rpm, dual Rushton-style impellers AGITATION Cell Culture 30 - 300 rpm, direct or magnetic drive, pitched blade impeller All vessels use P-I-D control for heating and cooling Control Heat-Blanketed Vessels: External heat mantle & high-capacity immersed cooling coil TEMPERATURE Water-Jacketed Vessels: Temperature-controlled water in glass jacket Maximum Temp. 70° C Primary Control Unit (PCU) plus optional, stackable loop-control modules. CONTROLLER DESIGN PCU supports 4 vessels — up to 32 loops, total Serial Port with AFS and Modbus protocols. 4-channel 0 - 2.5 V DC output for recorder COMMUNICATIONS Human Interface Control Center for up to 4 vessels Functions 1 Display Bright yellow text & graphics on self-illuminated, 3.4 x 4.5” (8.6 x 11.4 cm) ⁄4 VGA screen PRIMARY Multi-Vessel Automatically senses attached loop controllers Support CONTROL Upgrades Built-in floppy drive for easy firmware upgrades UNIT (PCU) Display Languages English, German, French & Spanish Control Modes Manual and Automatic Operating Modes Fermentation and Cell Culture Provides power for temperature control (heating and cooling), agitation and 5 optional POWER CONTROLLER pumps for liquid additon and removal 4-PUMP MODULE ◊ Module provides four peristaltic pumps for liquid addition and removal Module supports three conduction probes for automating liquid additions and ANTIFOAM / LEVELS removals, and foam control CONTROLLER ◊ General Info Optional controller with one pH and one dissolved oxygen probe connection DO - pH Dissolved Setpoint range: 0 - 100 %. Agitation or oxygen enrichment cascades. 2 CONTROLLER◊ Oxygen Control Sparge on demand. Four-gas algorithm for cell culture application Setpoint range: 2 - 12. Control - P-I-D with adjustable deadband. Acid/base pH Control liquid additions (requires pumps). Four-gas mixing (requires Gas Mix Controller) Four solenoid valves sequence additions of up to four gasses for pH and DO GAS MIXER ◊ control in cell culture, or oxygen enrichment of sparge gasses 85 - 265 VAC, 50 - 60 Hz PCU POWER Power Controller 115/230 VAC, 50 - 60 Hz REQUIRE- 4-Pump Module 115 VAC, 60 Hz or 230 VAC, 50 Hz, as labeled MENTS Loop Controllers 12 V DC (supplied via interconnect cable) CERTIFICATIONS CE, cETL Complete Basic and Advanced Fermentation and Cell Culture Kits are available, or SIMPLIFIED ORDERING components may be ordered individually. See Product Catalog/Price List for details (*) Specifications subject to change without notice. (◊) Not all controllers are included with all kits. Refer to Price List/Product Catalog for details. VESSEL DIMENSIONS, AS PREPARED FOR AUTOCLAVE NET WEIGHT, COMPLETE KITS NON-JACKETED VESSELS Fermentation Cell Culture VESSEL With Exhaust Condenser Without Exhaust Condenser Vessel Size Basic Kit Advanced Kit Avanced Kit Total Length Width Height Diameter Height Type T.V. lbs kg lbs kg lbs kg Volume Inches cm Inches cm Inches cm Inches cm Inches cm l.3 37 17 62 28 67 30 1 1 1 1 1.3 & 3 L 9 24 8 21 22 58 8 21 16 41 ⁄2 ⁄2 ⁄2 ⁄2 3 43 19 68 31 73 33 Non- 1 1 1 1 1 7.5 L 14 36 11 29 25 66 11 29 19 49 ⁄2 ⁄2 ⁄2 ⁄2 ⁄2 Jacketed 7.5 63 28 88 40 93 42 1 1 1 14L 11 29 11 29 29 75 11 29 24 60 ⁄2 ⁄2 ⁄2 14 67 31 92 42 97 44 On Optional Slant Rack l.3 39 18 64 29 69 31 1 7.5 L 28 71 11 29 18 46 — — — — ⁄2 Water- 3 43 20 68 31 73 33 1 1 14 L 31 79 11 29 20 50 — — — — ⁄2 ⁄2 Jacketed 7.5 64 29 89 41 94 43 WATER-JACKETED VESSELS 14 75 34 100 46 105 48 VESSEL With Exhaust Condenser Without Exhaust Condenser Total Length Width Height Diameter Height Vessel size in the charts above and at left indicate total volume. For Volume shipping weight for the 1.3 & 3 L kits, add approximately 44 lbs. (20 Inches cm Inches cm Inches cm Inches cm Inches cm 1 kg); for larger kits, add 55 lbs. (25 kg.) 1 1 1 1.3 L 11 29 9 24 20 52 9 ⁄4 24 16 41 ⁄2 ⁄2 ⁄2 1 1 1 1 3 L 11 29 9 24 22 56 9 ⁄4 24 18 45 ⁄2 ⁄2 ⁄2 1 1 1 1 1 7.5 L 11 29 11 29 24 63 11 29 20 52 ⁄2 ⁄2 ⁄2 ⁄2 ⁄2 1 1 1 14 L 11 29 11 29 31 79 11 29 27 69 ⁄2 ⁄2 ⁄2 On Optional Slant Rack 1 1 7.5 L 26 66 11 29 18 47 — — — — ⁄2 ⁄2 1 1 1 14 L 28 72 11 29 20 52 — — — — ⁄2 ⁄2 ⁄2 New Brunswick Scientific Co. Inc. PO Box 4005 • 44 Talmadge Rd. • Edison, NJ 08818-4005 800-631-5417 • 732-287-1200 • Fax 732-287-4222 www.nbsc.com • bioinfo@nbsc.com